孟昱,汪晴,蔡蕊,李梅,孟庆刚,赵伟杰.保健品中枸橼酸西地那非成分近红外快速定性定量分析[J].,2010,(6):867-870 |
保健品中枸橼酸西地那非成分近红外快速定性定量分析 |
Quantitative and qualitative analyses of Sildenafil Citrate in healthy products using near infrared spectroscopy |
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DOI:10.7511/dllgxb201006005 |
中文关键词: 近红外 液体透射 枸橼酸西地那非 保健品 |
英文关键词: near infrared liquid transmittance Sildenafil Citrate healthy product |
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中文摘要: |
建立了应用近红外光谱技术对保健品中枸橼酸西地那非成分的定量及定性分析方法.采集36个样品的液体透射光谱,分别采用主成分分析法和第一范围标定法建立定量和定性模型,并将定量分析方法的结果与HPLC定量结果进行比对.所建定量分析模型,主成分数为4, R 2 为99.92%,RMSECV为31.5.该模型对预示集的枸橼酸西地那非HPLC平均方法回收率为104.8%,近红外光谱分析法处理结果与HPLC法处理结果无显著差异.定性分析模型组间选择性为4.38,对18个未知样品判错率为0.所建立的新方法可对保健品中枸橼酸西地那非成分进行快速准确的定性及定量分析. |
英文摘要: |
The quantitative and qualitative analyses of healthy product with Sildenafil Citrate using near infrared spectroscopy (NIR) were developed. 36 samples were analyzed using NIR. The mathematical models of quantitative and qualitative analyses were developed by principal component regression (PCA) method and scaling to the first range method, respectively. The quantitative results between HPLC and NIR were compared. The principal component regression is 4, the correlation coefficient (R 2) of the training set validated by cross-validation is 99.92%, RMSECV is 31.5. The quantitative analysis models were used to determine the samples used for validation, and the average recoveries of Sildenafil Citrate was 104.8%. There is no significant difference between NIR and HPLC method. The selectivity between groups in quantitative analysis model is 4.38,and mistaken judgment rate of 18 samples is 0. This new method can be applied to quantitative and qualitative analyses of Sildenafil Citrate in healthy products. |
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