| 王竞,苏妍彦,李丽华,吕红,金若菲,周集体.共固定化醌还原菌群与蒽醌促进偶氮染料生物脱色[J].,2011,(2):174-179 |
| 共固定化醌还原菌群与蒽醌促进偶氮染料生物脱色 |
| Enhanced biodecolorization of azo dyes by co-immobilization of quinone-reducing consortium and anthraquinone |
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| DOI：10.7511/dllgxb201102004 |
| 中文关键词: 醌还原菌群 蒽醌 共固定化 偶氮染料 生物脱色 |
| 英文关键词: quinone-reducing consortium anthraquinone co-immobilization azo dyes biodecolorization |
| 基金项目:国家自然科学基金资助项目(50578022;50978040). |
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| 中文摘要: |
| 水溶性氧化还原介体可加速偶氮染料厌氧生物脱色,但会随出水而流失,造成二次污染．将醌还原菌群与非水溶性介体——蒽醌共固定化,考察了其对偶氮染料生物脱色的影响．克隆和RIS片段测序结果表明,醌还原菌群主要包括乳杆菌科 (Lactobacillaceae)、肠杆菌科(Enterobacteriaceae)和拟杆菌科(Bacteroidaceae)； 共固定化醌还原菌群与蒽醌对10种不同结构偶氮染料的生物脱色速率提高1.3~2.1倍；共固定化菌球经10次循环使用后,对活性艳红KE-3B脱色率仍保持在85%以上． |
| 英文摘要: |
| The decolorization rate of azo dyes under anaerobic conditions can be improved by the addition of water-soluble redox mediators. However, the continuous dosing and discharge of water-soluble redox mediators, such as biologically recalcitrant compounds, will result in the secondary contamination. The accelerating effect of co-immobilization of quinone-reducing consortium and insoluble anthraquinone in the biodecolorization of azo dyes was investigated for the first time. The cloning and sequencing analysis of ribosomal intergenic spacer (RIS) genes show that quinone-reducing community is predominate by Lactobacillaceae, Enterobacteriaceae and Bacteroidaceae; the decolorization rates of a broad range of azo dyes are greatly improved by co-immobilized beads as 1.3-2.1-fold increases; after ten repeated experiments, the decolorization rate of Active Red KE-3B by co-immobilized beads retains over 85%. |
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