| 关水,刘天庆,葛丹,陆瑞欣,马学虎,崔占峰.胶原-壳聚糖复合支架体外三维培养神经干细胞[J].,2011,(5):637-643 |
| 胶原-壳聚糖复合支架体外三维培养神经干细胞 |
| Collagen-chitosan composite scaffold in vitro three-dimensional culture of neural stem cells |
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| DOI：10.7511/dllgxb201105003 |
| 中文关键词: 神经干细胞 三维培养 复合支架 胶原-壳聚糖 |
| 英文关键词: neural stem cells three-dimensional culture composite scaffold collagen-chitosan |
| 基金项目:国家自然科学基金资助项目(30800288,30670525)；教育部高等学校博士学科点新教师基金资助项目(20070141050)；大连理工大学科研启动基金资助项目(1000893364). |
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| 中文摘要: |
| 采用冷冻干燥法制备了胶原-壳聚糖复合支架并通过层粘连蛋白(LN)进行结构修饰,测定其物理性能特征,建立了胎鼠海马神经干细胞(NSCs)的三维培养体系,同时考察了NSCs与胶原-壳聚糖复合支架的生物相容性．孔径、孔隙率、吸水率、降解率等参数的比较表明,体积比为7∶3的复合支架更适合于体外细胞培养的要求,并且NSCs在经LN修饰后支架材料上的接种率得到明显提高；激光共聚焦显微镜及扫描电镜观察表明,NSCs能够在胶原-壳聚糖复合支架内良好地生长、增殖及分化．这些结果为NSCs的进一步临床移植应用,以及治疗神经系统疾病的新药筛选和机理研究等奠定了坚实的实验基础． |
| 英文摘要: |
| The collagen-chitosan composite scaffolds were fabricated by lyophilization technique, which were structurally modified by laminin (LN) protein, and their physical performance characteristics were determinated. Neural stem cells (NSCs) obtained from the fetal mouse hippocampus were cultured in the collagen-chitosan composite scaffolds and the biocompatibility was studied. The comparative results of different scaffolds with the parameters of aperture, porosity, water absorption and degradation rate show that the volume ratio of 7∶3 is more suitable for cell culture in vitro and the inoculation rate of NSCs is markedly improved in LN-treated scaffolds. Through the observation of laser scanning confocal microscope (LSCM) and scanning electron microscopy (SEM), NSCs within collagen-chitosan composite scaffolds present good growth, proliferation and differentiation. These results provide a scientific experimental basis not only for further NSCs clinical transplantation applications, but also for the drug screening and mechanism research on the treatment of diseases of the nervous system. |
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