| 曲媛媛.两相体系中固定化联苯双加氧酶全细胞产靛蓝特性研究[J].,2013,53(3):340-345 |
| 两相体系中固定化联苯双加氧酶全细胞产靛蓝特性研究 |
| Research on characteristics of biotransformation of indigo by immobilized whole cell expressing biphenyl dioxygenase in biphasic system |
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| DOI：10.7511/dllgxb201303005 |
| 中文关键词: 联苯双加氧酶 基因工程菌 靛蓝 海藻酸钠 两相体系 |
| 英文关键词: biphenyl dioxygenase gene engineering bacteria indigo sodium alginate biphasic system |
| 基金项目:国家自然科学基金资助项目(21176040,51078054) |
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| 摘要点击次数: 1229 |
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| 中文摘要: |
| 微生物全细胞转化靛蓝的过程中,靛蓝易被游离细胞吸附,造成产物提取困难,是微生物产靛蓝工业化进程中面临的主要问题之一．为解决该问题,尝试了不同固定化方法将基因工程菌固定,其中海藻酸钠包埋法取得了最好的效果,最佳包埋条件为3.5%海藻酸钠、1%氯化钙,固定化细胞在有机\|水两相体系中可以重复利用两次,首次转化6 h后,靛蓝浓度为15.9 mg/L．另外,两相体系的引入有效避免了固定化细胞产靛蓝过程中伴随的产物分解现象,选择正十二烷作为有机相,优化得到其最佳比例为体积分数40%．体系对吲哚的最佳耐受浓度为400 mg/L,在此条件下,靛蓝产率为0.28 mg/mg． |
| 英文摘要: |
| The extraction of indigo is delayed due to the adsorption of indigo by the free whole cell, which is one of the major difficulties during the biotransformation of indigo. Three immobilization methods are used, and sodium alginate immobilization method obtains the best results. The immobilized cell can be repeatedly used for two times in a biphasic system under the optimum immobilization condition:3.5% sodium alginate, 1% CaCl2, and the indigo production is 15.9 mg/L after 6 h of the first transformation. Besides, the indigo decomposition can be avoided by the biphasic system with 40% dodecane as the optimal organic phase. The optimum indole tolerance concentration is 400 mg/L, and the productivity of indigo is 0.28 mg/mg. |
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