| 张旭旺.苯酚羟化酶生物转化吲哚及甲基吲哚研究[J].,2013,53(3):346-353 |
| 苯酚羟化酶生物转化吲哚及甲基吲哚研究 |
| Research on biotransformation of indole and methylindoles by phenol hydroxylase |
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| DOI：10.7511/dllgxb201303006 |
| 中文关键词: 吲哚 甲基吲哚 苯酚羟化酶 表面响应法 |
| 英文关键词: indole methylindoles phenol hydroxylase response surface methodology |
| 基金项目:国家自然科学基金资助项目(51078054,21176040,20923006) |
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| 中文摘要: |
| 利用苯酚羟化酶基因工程菌PH_IND对吲哚及甲基吲哚进行生物转化.结果表明,除了3-甲基吲哚外,PH_IND能将吲哚、2-甲基吲哚、4-甲基吲哚和7-甲基吲哚转化成靛蓝类色素．采用薄层色谱和液相-质谱等手段对转化产物进行鉴定,结果表明,PH_IND转化吲哚和4\|甲基吲哚得到的蓝色产物分别为靛蓝和4,4′-二甲基靛蓝,并推测了PH_IND转化吲哚及4-甲基吲哚的反应途径．采用表面响应法对PH_IND转化吲哚、2-甲基吲哚及4-甲基吲哚的条件进行了优化,在最优条件下,菌株PH_IND转化吲哚合成靛蓝的最大产量为93.74 mg/L,其吲哚的转化效率为52.63%,且菌株PH_IND对2-甲基吲哚和4-甲基吲哚的最大转化率分别为67.66%和95.61%． |
| 英文摘要: |
| The gene engineering strain PH_IND expressing phenol hydroxylase is used to catalyze the
biotransformation of indole and methylindoles. The experimental results indicate that indole, 2-methylindole, 4-methylindole and 7-methylindole can be transformed into various indigoid pigments except for 3-methylindole. The predominant color products are identified by thin-layer chromatography and liquid chromatography-mass spectrometry analyses, and the major products (blue pigment) derived from indole and 4-methylindole are authenticated as indigo and 4, 4′-dimethylindigo, respectively. Meanwhile, the biotransformation pathways of indole and 4-methylindole by PH_IND are proposed. Response surface methodology is applied to optimize the biotransformation conditions of indole, 2-methylindole and 4-methylindole by PH_IND. Under the optimal conditions, the indigo yield and transformation efficiency of indole are 93.74 mg/L and 52.63%, respectively, while the maximum transformation rates of 2-methylindole and 4-methylindole are 67.66% and 95.61%, respectively. |
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