| 金若菲.低强度超声对Rhodotorula mucilaginosa Z1降解硝基苯的强化作[J].,2013,53(5):637-641 |
| 低强度超声对Rhodotorula mucilaginosa Z1降解硝基苯的强化作 |
| Enhancement of nitrobenzene degradation with Rhodotorula mucilaginosa Z1 by low intensityultrasound |
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| DOI：10.7511/dllgxb201305003 |
| 中文关键词: 低强度超声 生物降解 硝基苯 Rhodotorula mucilaginosa Z1 |
| 英文关键词: low intensity ultrasound biodegradation nitrobenzene Rhodotorula mucilaginosa Z1 |
| 基金项目:国家科技重大科研专项资金资助项目(2012ZX07202-002) |
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| 中文摘要: |
| 考察超声作用对其降解能力、生长量、酶活及细胞膜通透性的影响．对于初始浓度为589.5 mg/L的硝基苯,在前8 h中超声组和对照组的降解率分别为44.2%和25.4%,最终去除率都可达到90%以上,说明低强度超声可以提高生物的适应性,提高降解速率．经测定,超声组释放的粗酶较对照组增加了10%,超声组的2-氨基苯酚-1,6-双加氧酶的活性比对照组高出8.8%,说明低强度超声提高了酶活．另外,在R. mucilaginosa Z1细胞内、外,超声组的硝基苯浓度均低于对照组,初始浓度为523.8 mg/L的细胞外硝基苯浓度,超声组比对照组低8.9 %,说明超声作用改变了细胞膜的通透性． |
| 英文摘要: |
| Low intensity ultrasound(LIU) was used to enhance the microbial degradation of nitrobenzene (NB) with
Rhodotorula mucilaginosa Z1 (R. mucilaginosa Z1). The parameters of ultrasound treatment on NB degradation were determined to be 25 kHz, 180 W, 25 ℃ and 15 min. The effects of LIU treatment on degradation of NB, growth of R. mucilaginosa Z1, enzyme activity of the strain and cell membrane permeability were studied. The degradation efficiencies of LIU-treated and control groups for NB (589.5 mg/L) in the initial 8 h are 44.2% and 25.4%, respectively. Both groups can reach 90% removal of NB at the end of the investigation, indicating that LIU treatment can improve the adaptability of the strain and enhance the degradation rate. The amount of crude enzyme detected in LIU-treated group is 10% higher than that in the control group. The 2-aminophenol-1,6-dioxygenase activity of the strain in LIU-treated group is 8.8% higher than that in the control group, suggesting that LIU treatment stimulates the enzyme activity of the strain. In addition, both intra- and extracellular NB concentrations in LIU-treated group are lower than those in the control group. The extracellular NB
concentration in LIU-treated group is 8.9% lower than that in the control group when the initial NB concentration is 523.8 mg/L, which suggests that cell membrane permeability is changed by LIU treatment. |
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