| 杨青,潘悦,肖桂山.miR-23b对口腔癌转移影响[J].,2020,60(1):10-14 |
| miR-23b对口腔癌转移影响 |
| Effect of miR-23b on oral cancer metastasis |
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| DOI：10.7511/dllgxb202001002 |
| 中文关键词: 口腔癌 miR-23b 口腔癌转移 标志物 |
| 英文关键词: oral cancer miR-23b oral cancer metastasis marker |
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| 中文摘要: |
| 口腔癌转移是威胁全世界生命健康的问题，有效的靶向治疗对口腔癌患者尤为重要．近年来，microRNA(miRNA)作为一种微小的非编码RNA在肿瘤转移中发挥着重要的作用，因此，开展了体外探究miR-23b在口腔癌转移中的作用．利用实时荧光定量核酸扩增检测系统(qRT-PCR)检测了miR-23b在口腔正常细胞和口腔癌细胞中的表达差异，结果显示miR-23b在口腔癌细胞中的表达明显高于口腔正常细胞中的表达，且随着口腔癌转移程度的增加而增高(P<0.05)．在此基础上，通过给细胞转染miR-23b mimics的方法过表达miR-23b，以探究该miRNA对口腔癌转移的作用．实验结果发现，增加口腔原位癌细胞Um-2中miR-23b的表达后，该细胞的转移能力显著增强，表明miR-23b在口腔癌转移中发挥着重要作用，可能成为临床口腔癌的治疗靶点和口腔癌检测标志物． |
| 英文摘要: |
| Oral cancer metastasis is a problem that threatens life around the world. Effective targeted therapy is especially important for patients with oral cancer. In recent years, it is found that microRNA (miRNA) plays an important role as a tiny non-coding RNA in tumor metastasis. For this reason, an in-vitro study of the role of miR-23b in oral cancer metastasis is carried out. Real-time fluorescent quantitative nucleic acid amplification detection system (qRT-PCR) is used to detect the differential expression of miR-23b in normal oral cells and oral cancer cells, and it is found that the expression of miR-23b in oral cancer cells is significantly higher than that in normal oral cells, and increases with the increase of oral cancer metastasis (P<0.05). Further, the effect of this miRNA on oral cancer metastasis is investigated by transfecting miR-23b mimics to increase the expression of miR-23b. The experimental results show that the expression of miR-23b in Um-2 cells of oral cancer cells is increased, and the metastatic ability of the cells is significantly enhanced, which indicates that miR-23b plays an important role in oral cancer metastasis and may become a therapeutic target for clinical oral cancer and a new marker for oral cancer detection. |
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