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| 脱氮硫杆菌利用FeS自养反硝化过程研究 |
| Study on Autotrophic Denitrification Process Conducted by Thiobacillus denitrificans Utilizing FeS |
| 投稿时间：2019-03-30 修订日期：2019-04-24 |
| DOI： |
| 中文关键词: 脱氮硫杆菌 FeS 自养反硝化 反应动力学 |
| 英文关键词: Thiobacillus denitrificans iron sulfide autotrophic denitrification reaction dynamics |
| 基金项目:国家自然科学(51078056, 51578106) |
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| 中文摘要: |
| 以硫化亚铁为电子供体的自养反硝化反应对水体中硝酸盐的去除有重要贡献。本文以菌株Thiobacillus denitrificans ATCC25259为对象，首次研究了脱氮硫杆菌以FeS为底物的自养反硝化过程。结果表明，以FeS作为唯一电子供体时，T. denitrificans可以将NO3--N（30 mg·L-1）彻底还原为N2。同时，FeS中的硫元素经自养反硝化过程转化为SO42-，而铁元素与培养基中PO4-反应生成沉淀物Fe3(PO4)2·8H2O。通过对相关数据拟合发现NO3-和NO2-的还原过程遵循零级反应动力学(R2 > 0.93)，随着FeS加入量的增加，NO3-和NO2-的还原速率均增加，但NO3-的还原速率增加更多，使中间产物NO2-的积累量增大。 |
| 英文摘要: |
| The autotrophic denitrification reaction with iron sulfide as the electron donor plays a significant role in nitrate removal in water body. In this study, the strain Thiobacillus denitrificans ATCC25259 was novelly applied to investigate the process of autotrophic denitrification with FeS as substrate. The results exihibited that NO3--N (30 mg.L-1) was finally reduced to N2 by T. denitrificans utilizing FeS as the sole electron donor. The elemental sulphur in FeS was converted to SO42- via autotrophic denitrification, wheras the elemental iron was precipitated with PO4- as the form of Fe3(PO4)2.8H2O. Besides, according to the fitted curves, this autotrophic denitrification process was demonstrated to accord with the zero-order kinetics (R2 > 0.93). As the rising amount of FeS, both the reduction rates of NO2- and NO3- increased. Additionally, the reduction rate of NO3- grew even more sharply, which accounted for the improved accumulation concentration of NO2- as intermediates. |
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