| 金礼吉.新型双萘酰亚胺类化合物与DNA作用研究[J].,2013,53(2):169-175 |
| 新型双萘酰亚胺类化合物与DNA作用研究 |
| Research on interaction of novel bis\|naphthalimide derivatives with DNA |
| 投稿时间：2013-03-04 修订日期：2013-03-05 |
| DOI：10.7511/dllgxb201302003 |
| 中文关键词: 双萘酰亚胺 嵌入剂 荧光光谱 圆二色光谱 |
| 英文关键词: bis-naphthalimide intercalator fluorescence spectra circular dichroism spectra |
| 基金项目:大连理工大学理学学科基金资助项目（SFDUT07007） |
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| 中文摘要: |
| 运用紫外吸收光谱、荧光光谱和圆二色光谱等技术方法,研究了5种不同长短烷基异硫脲阳离子作为连接臂的新型双萘酰亚胺类化合物(DN2~DN6)与DNA的作用．紫外吸收光谱结果表明,该类化合物能使DNA发生明显的减色效应并伴有微弱的红移现象,其减色效应随化合物烷基链的延长而增强；化合物的荧光可被DNA强烈猝灭,室温下DN3~DN6与DNA的结合常数随烷基链的延长而增大,分别为1.18×105、1.36×105、1.67×105、2.32×105 L/mol；化合物能够将嵌入在DNA中的吖啶橙游离出来,烷基链越长的化合物该作用力也越强；圆二色光谱表明,DNA的双螺旋结构发生了碱基堆积和局部解旋,碱基堆积程度与化合物烷基链的长度成正相关．实验结果表明,DN2~DN6均以嵌入方式与DNA发生作用,其作用强度顺序为DN6>DN5>DN4>DN3>DN2 |
| 英文摘要: |
| The interaction of the five novel bis\|naphthalimide derivatives (DN2\|DN6) linked by methyl\|isothiourea cation with DNA is investigated by UV absorption spectra, fluorescence spectra and circular dichroism (CD) spectra. The experimental results from UV absorption spectra show that when DNA is added, the hypochromic effect and faint red shift are observed in the absorption peak of DN2\|DN6, and the longer the linker is, the more obvious the hypochromic effect is. The fluorescence of the compounds can be strongly quenched by DNA. The binding constants between DN3\|DN6 and DNA increase with the alkyl chain′s extension, they are 1.18×105, 1.36×105, 1.67×105, 2.32×105 L/mol, respectively. The compounds can free the acridine orange which intercalates into DNA base pairs, and the force becomes stronger with the linker′s extension. The CD spectrum results show that the double helix structure of DNA comes forth base stacking and unwinding locally, and the degree of base stacking is positively correlated with the length of alkyl chain. All experimental results show that DN2\|DN6 bound to DNA by the intercalating binding mode and the order of intensity is DN6>DN5>DN4>DN3>DN2 |
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