| 吕菁萍,李泽龙,张鹤睿,王竞.海洋假交替单胞菌生物膜产ROS特性研究[J].,2021,61(6):569-575 |
| 海洋假交替单胞菌生物膜产ROS特性研究 |
| Study of ROS production characteristics of biofilm by marinePseudoalteromonas sp. |
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| DOI：10.7511/dllgxb202106003 |
| 中文关键词: 活性氧 生物膜 假交替单胞菌 海洋环境 |
| 英文关键词: reactive oxygen species biofilm Pseudoalteromonas sp. marine environment |
| 基金项目:国家自然科学基金资助项目(21876018). |
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| 摘要点击次数: 219 |
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| 中文摘要: |
| 海洋微生物可以产生胞外活性氧(reactive oxygen species，ROS)，为深海无光区提供了活性氧．而在实际海洋环境中，海洋微生物更倾向于以生物膜形式生长，但生物膜形态对菌体产ROS的影响鲜见报道．考察了海洋假交替单胞菌(Pseudoalteromonas sp.)GCY生物膜产ROS特性．结果表明，GCY生物膜胞外h2O2、O-2以及?OH等产量均高于游离菌体，其中生物膜体系h2O2产量及O-2产量最高可达到游离菌体体系的3.10倍和3.56倍．同时L-氨基酸氧化酶(LAAO)、脱氢酶活性(DHA)、电子传递系统活性(ETSA)均有所提高．以生物膜形式生长的GCY与游离态GCY相比，胞外聚合物组分中多糖和蛋白质含量均有提升，胞外聚合物(EPS)总量增加了149 mg·L-1，增强了细胞黏附性并减少了ROS对细胞造成的损伤． |
| 英文摘要: |
| Marine microorganisms, which can produce extracellular reactive oxygen species (ROS), are inferred as source of ROS for marine aphotic zones. However, in the actual marine environment, microorganisms have the tendency to form into biofilms, and the effect of biofilm on ROS production has rarely been reported. So ROS production characteristics of Pseudoalteromonassp. GCY biofilm are investigated. According to the results, the extracellular h2O2, O-2 and?OH production of GCY biofilm is higher than that of free cells. Extracellular h2O2 production and extracellular O-2 production are 3.10 times and 3.56 times higher than that of free cells, respectively. Meanwhile, the activities of L-amino acid oxidase (LAAO), dehydrogenase activity (DHA) and electron transport system activity (ETSA) are also increased. Compared with free cells, polysaccharide and protein contents in extracellular polymer substance (EPS) components are both increased. The total EPS production increases by 149 mg·L-1, which enhances cell adhesion and reduces ROS induced damage to cells. |
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