| 吴硕,张兴文,吕菁萍,王竞.假交替单胞菌GCY生物膜降解双酚A研究[J].,2022,62(3):238-245 |
| 假交替单胞菌GCY生物膜降解双酚A研究 |
| Research on degradation of bisphenol A bybiofilm of Pseudoalteromonas sp. GCY |
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| DOI：10.7511/dllgxb202203003 |
| 中文关键词: 假交替单胞菌 生物膜 双酚A 活性氧 海洋环境 |
| 英文关键词: Pseudoalteromonas sp. biofilm bisphenol A reactive oxygen species marine environment |
| 基金项目:国家自然科学基金资助项目(21876018). |
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| 中文摘要: |
| 双酚A(bisphenol A，BPA)是一种海洋环境中普遍检出的内分泌干扰物，但生物膜作为海洋细菌最常见的生存方式，其对海洋环境中BPA归趋的影响尚不清晰．考察了典型海洋细菌——假交替单胞菌GCY的生物膜对BPA的降解特性及降解途径．结果表明，生物膜及游离菌对BPA降解均符合准一级反应动力学．生物膜体系中BPA的降解速率常数为0.042 5 h-1，大于游离菌体系的0.037 4 h-1．并证实两体系对BPA的降解均发生在胞外，以活性氧(reactive oxygen species，ROS)介导的降解反应为主．降解过程中生物膜体系的H2O2、O-2、OH产量高于同时刻游离菌体系的，这归因于生物膜体系L-氨基酸氧化酶活性及铁载体产量的提高．在生物膜体系中，BPA的主要降解途径为异丙基断裂． |
| 英文摘要: |
| Bisphenol A (BPA) is an endocrine disrupting chemical widely detected in the marine environment. Biofilm is regarded as the most common existence form of marine bacteria, however, its impact on the fate of BPA in the marine environment is still unclear. The degradation characteristics and degradation pathways of BPA by the biofilm of Pseudoalteromonas sp. GCY are investigated. The study results show that the degradation of BPA by the biofilm and the free cells fits the pseudo-first order kinetics. The degradation rate constant of BPA in the biofilm system is 0.042 5 h-1, which is higher than that of 0.037 4 h-1of the free cells system. It is confirmed that the degradation of BPA in two systems occurs in the extracellular fraction and is mainly mediated by reactive oxygen species (ROS). During the degradation process, the H2O2, O-2, OH production of the biofilm system is higher than that of the free cells system, which can be attributed to the increase of L-amino acid oxidase activity and siderophores production. In the biofilm system, the main degradation pathway of BPA is isopropyl rupture. |
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