| 王思,曲玥阳,肖桂山.SLC44A5对肺腺癌细胞迁移影响[J].,2022,62(6):570-575 |
| SLC44A5对肺腺癌细胞迁移影响 |
| Effect of SLC44A5 on migration of lung adenocarcinoma cells |
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| DOI：10.7511/dllgxb202206003 |
| 中文关键词: 肺腺癌 SLC44A5 细胞增殖 细胞迁移 |
| 英文关键词: lung adenocarcinoma SLC44A5 cell proliferation cell migration |
| 基金项目:国家自然科学基金资助项目(81770846)；中国博士后科学基金资助项目(2020M670760). |
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| 中文摘要: |
| 肺癌是发病率和死亡率最高的恶性肿瘤．SLC44A5是一种胆碱转运蛋白，其与肿瘤关系的相关报道较少．有报道显示其与肝癌的发生发展有关，然而其在肺癌中的作用尚未有相关报道．为探讨SLC44A5的影响，使用人肺腺癌细胞系H1299和H1792构建SLC44A5低表达细胞系，利用MTT法证明敲低SLC44A5会抑制细胞增殖．使用细胞划痕实验和Transwell细胞迁移实验证明敲低SLC44A5抑制细胞迁移．结果表明SLC44A5可能参与了肺癌的转移，具有成为肿瘤治疗分子靶点的潜力． |
| 英文摘要: |
| Lung cancer is the malignant tumor with the highest morbidity and mortality. SLC44A5 is a choline transporter, and there are few reports about its relationship with tumors. There are reports showing that it is related to the occurrence and development of liver cancer, but its role in lung cancer has not yet been reported. To research the effect of SLC44A5, human lung adenocarcinoma cell lines H1299 and H1792 are used to construct shSLC44A5 cell line, and MTT method is used to prove that SLC44A5 knockdown could inhibit cell proliferation. Using cell scratch and Transwell cell migration experiments proves that knocking down SLC44A5 inhibits cell migration. These results suggest that SLC44A5 may be involved in the metastasis of lung cancer and has the potential to become a molecular target for tumor therapy. |
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