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| 荧光/比色双模式检测体系用于苯二酚同分异构体的区分 |
| Dual-mode fluorescent/colorimetric detection method for the discrimination of dihydroxybenzene isomers |
| 投稿时间：2024-03-15 修订日期：2024-04-19 |
| DOI： |
| 中文关键词: 苯二酚, 比色检测, 双模式检测，荧光检测，纳米酶 |
| 英文关键词: dihydroxybenzene, colorimetric detection, dual-mode detection, fluorescent detection, nanozyme. |
| 基金项目:中央高校基本科研业务费( DUT22JC03 )资助. |
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| 中文摘要: |
| 邻苯二酚(CC)、间苯二酚(RC)和对苯二酚(HQ)是苯二酚的三种同分异构体,被广泛应用于化妆品、纺织、橡胶、医药等行业,研究表明CC、RC以及HQ对人体的毒理作用不同,因此,对三种苯二酚的分类鉴定对于保证人类健康非常重要。本研究将荧光和比色分析相结合,开发了一种简便的苯二酚双模式检测体系。在研究中,制备得到了具有光致发光特性和类氧化物酶活性的MnO2@N-GQD复合材料。MnO2@N-GQD可以催化氧化无色的邻苯二胺(OPD)生成淡黄色具有荧光特性的二氨基吩嗪(DAP)。当存在苯二酚时,DAP的生成受到抑制,导致检测体系的颜色和荧光强度发生改变,可据此构建基于荧光/比色技术的苯二酚双模式检测方法,实现了荧光模式和比色模式下对CC、RC和HQ的线性定量检测。在主成分分析的辅助下,将比色信号和荧光信号共同作为参数,实现了三种苯二酚的分类识别。使用该方法成功地实现了污水样品中CC、RC、HQ中三种苯二酚的分类。本研究为三种苯二酚同分异构体的分类识别提供了新的思路,具有一定的应用前景。 |
| 英文摘要: |
| Catechol (CC), resorcinol (RC) and hydroquinone (HQ) are three isomers of dihydroxybenzene, which are widely used in cosmetics, textile, rubber, medicine and so on. Studies have shown that CC, RC and HQ have different toxic effects on the human body. Therefore, the classification and identification of three catechol are very important for ensuring human health. In this study, a simple dual-mode detection system for dihydroxybenzene was developed by combining fluorescence and colorimetric analysis. Here, MnO2@N-GQD composites with photoluminescent properties and oxidase-like activity were prepared. MnO2@N-GQD can catalyze the oxidation of colorless O-phenylenediamine (OPD) to produce light yellow diaminophenazine (DAP) with fluorescent properties. In the presence of dihydroxybenzene, the generation of DAP is inhibited, resulting in changes in the color and fluorescence intensity of the detection system. Thus, a dual-mode detection method for dihydroxybenzene based on fluorescence/colorimetric technology can be constructed to achieve linear quantitative detection of CC, RC and HQ in both fluorescence mode and colorimetric mode. With the aid of principal component analysis, colorimetric signal and fluorescence signal were used together as parameters to realize the classification and recognition of three kinds of dihydroxybenzene. Using this method, the classification of CC, RC and HQ in sewage samples was successfully realized. This study provides a new idea for the classification of three isomers of dihydroxybenzene, and has a certain application prospect. |
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